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This suppression manifests as a 45% reduction in cyclic adenosine monophosphate (cAMP) levels and 53% inhibition of protein kinase A (PKA) activity, effectively disrupting the molecular machinery required for normal testicular function ( 3.2 Metabolic enzyme regulation and steroidogenesis EED interference with critical enzymatic pathways responsible for testosterone biosynthesis and xenobiotic detoxification creates profound hormonal imbalances that compromise reproductive function

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Subsequently, data were imported into the Galaxy platform maintained by GenAP ( and hosted by the Digital Research Alliance of Canada