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In the present study, RGSH reduced the level of iron ions and ACSL4 in AKI, and increased the expression of GPX4, thereby reducing the degree of kidney damage, which demonstrated that RGSH could ameliorate hemolytic AKI via regulation of the ferroptosis pathway, which was consistent with the results of previous studies that RGSH affects ferroptosis via regulation of the ferroptosis-related gene GPX4 (30)