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Cells were first stained with 50 µM DCFDA or 10 µM mBcl, as described above, followed by a washing step with PBS and a surface staining of 30 min on ice with combinations of the following monoclonal antibodies: PE-conjugated anti-CD10 (HI10α), PerCP-conjugated anti-CD3 (SK7), APC-conjugated anti-CD19 (SJ25C1), PE-conjugated anti-CD5 (L17F12), PE-conjugated anti-CD25 (PC61), all from BD Biosciences

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Thus, we established an in vitro CS culture system comprising RAW264.7 cells that could mimic the response of intrapulmonary macrophages to silicosis