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Fifteen minutes after adding a stopper solution, absorption of standards and samples at 450 nm wavelengths were read by ELISA (BioTeK-Synergy H1, USA)
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The mean (±SD) free carnitine concentration was 45.38 ± 12.61 µmol/L in the patient group, whereas it was 41.54 ± 9.85 µmol/L in the healthy control group, and the difference between the 2 groups generated a P value of 0.049
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